Objective: This study was to evaluate the effect of Vitamin C on the histology and histochemistry of the prefrontal cortex of ethanol-induced rats. Methods: Male Sprague- Dawley rats were used for the study. Ethical approval was obtained from the University�s ethical committee. The rats were randomly divided into 6 groups of 10 rats each. Rats in group A= free access to normal saline. Rats in group B= treated with 4.25 ml ethanol. Rats in group C= treated with 100 mg/kg Vit. C. Rats in group D= pre-treated with 100 mg/kg Vit. C followed by 4.25 ml ethanol. Rats in group E=co-treated with 100 mg/kg of Vit. C and 4.25ml ethanol. Rats in group F=post-treated with 4.25ml ethanol followed by 100 mg/kg Vit.C. 24hrs after the last administration, the rats were sacrificed by cervical dislocation: the fraction of the brain for tissue histochemistry was fixed in formol calcium and later processed for Heamotoxylin and Eosin with Cresyl fast violent staining techniques and the other fraction meant for enzyme and/or marker histochemistry was processed accordingly for some neurochemical indices for oxidative stress. Results: The markers of oxidative stress were statistically increased in the rats in group D, E and F compared with the rats in group B. There is a significant reduction of TBARS when compared with ethanol induced group (group B). The histological profile of the prefrontal cortex of rats in group A and C were preserved while that of the rats in group B displayed distorted cytoarchitecture profile with a marked increase in apoptotic bodies, lateral deviation of neurons and a marked increase in the activities of oxidative markers
This study seeks to investigate in a rat model the effect of vitamin E following reperfusion injury/testicular injury. Twenty (20) Wistar male rats weighing between 100-150g ranging in age from 4-7 weeks were used for this study and were grouped into five groups of four rats each. Group one was administered with intramuscular (IM) administration of vitamin E (200mg/kg bw) and undergone testicular torsion with reperfusion for an hour, group two rats had concurrent administration of vitamin E (200mg/kg bw) followed by testicular torsion with reperfusion for an hour, while group three undergone torsion and was treated with vitamin E (200mg/kg bw) after an hour of testicular torsion at 7200 clockwise rotation. Group four undergone testicular torsion for an hour after which detortion was done and reperfusion was allowed for another one hour. While group five served as the control rats. At the end of the experiment testes of all the treated rats were exposed and semen was obtained for sperm characteristics (sperm motility and count). The results showed that following testicular torsion, sperm motility was reduced significantly while sperm count was unaffected. Intramuscular administration of vitamin E following torsion showed insignificant decrease (p>0.05) in sperm function.
Plasmodium falciparum (Pf) has been found to be the deadliest of all the known species of the parasite capable of infecting humans; this is because it is capable of causing severe cerebral tissue damage. This study was carried out to demonstrate the parasite in the host blood in vitro through immunogold labeling using antibodies against Plasmodium falciparum histidine rich protein 2 (HRP 2); a major metabolite released during the cause of the parasite infection and feeding in the erythrocyte. 12 known Pf positive samples were obtained from across the six geopolitical zones of Nigeria and were further characterized by Geimsa thick and thin film for parasite identification parasite count expressed as parasites/l of blood. An average of 400 parasites/l of blood was obtained in each of the samples used for this study. Pf-HRP 2 antibody was conjugated to freshly prepared colloidal gold of particle size 40 nm. The conjugation process was blocked with bovine serum albumin (BSA) and the conjugate itself preserved by 1% glycerol and 0.01% sodium azide. The parasite count was titrated against the Pf-HRP 2 gold conjugate and was analyzed under the light microscope with a fluorescent filter. Reactivity and specificity of Pf-HRP 2 gold conjugate was found to be highly specific and gave direct identification of the erythrocytes infected with the parasite. A good contrast was also obtained between uninfected erythrocytes, parasite and the infected erythrocytes